Basic Fibroblasts Nucleofector® Kit

In addition to various cell type-specific kits for primary fibroblasts, we also provide a cell group-specific Basic Nucleofector™ Kit. This kit is suited to determine the optimal transfection conditions for any mammalian fibroblast type that lacks a cell type specific Optimized Protocol for the Nucleofector™ 2b Device (or older generations I or II). In a first experiment a selection of different Nucleofector™ Programs is tested in combination with the Basic Nucleofector™ Solution for fibroblasts. The respective cell type-specific Optimized Protocols or recommendations are available under "Instructions" or in our knowledge database. In these Optimized Protocols the best Nucleofection conditions are indicated. In addition, we share our experience and knowledge for trea™ent of individual cell types. Benefits Optimization can be performed within 1 experiment Detailed protocols guiding through the optimization procedure Optional result fine tuning with help of our Scientific Support Team Transfection efficiencies up to 90% Viabilities up to 98% Applications Kit suited for fibroblasts from different mammalian species and various organs Already tested for macaque dermal fibroblasts bovine fibroblasts human colon myofibroblasts mouse lung fibroblasts etc. Content & Storage Content 2.25 mL Nucleofector™ Solution for Primary Mammalian Fibroblasts 0.5 mL Supplement 1 30 µg pmaxGFP™ Vector (0.5 μg/μl in 10 mM Tris pH 8.0) 25 Aluminum cuvette (100 µL) 25 Single use pipettes The kit is available in different variants for either 10 or 25 or 4 x 25 reactions.