100 RCT Basic Glial Cells Kit

In addition to various cell type-specific kits for primary epithelial cells, we also provide a cell group-specific Basic Nucleofector™ Kit. This kit is suited to determine the optimal transfection conditions for any mammalian epithelial cell type that lacks a cell type specific Optimized Protocol for the Nucleofector™ 2b Device (or older generations I or II). In a first experiment a selection of different Nucleofector™ Programs is tested in combination with the Basic Nucleofector™ Solution for epithelial cells. The kit is available in different variants for either 10 or 25 or 4 x 25 reactions. The respective cell type-specific Optimized Protocols or recommendations are available under "Instructions" or in our knowledge database. In these Optimized Protocols the best Nucleofection conditions are indicated. In addition, we share our experience and knowledge for trea™ent of individual cell types. Benefits: Optimization can be performed within one experiment. Detailed protocols guide through optimization procedure . Fine tuning of results is possible with the help of our Scientific Support Team . Transfection efficiency up to 60%. Viability up to 80% Applications: Kit suited for various glial cells from different mammalian species. Already tested for rat and mouse astrocytes rat oligodendrocytes